Enzymatics试剂产品信息 EnzymaticsRNA酶
来源:    发布时间: 2018-05-16 14:29   32 次浏览   大小:  16px  14px  12px
蚂蚁淘(ebiomall

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Enzymatics是分子生物学试剂和制造服务的L先供应商,提供了无与伦比的质量,*性和价值的商业基因组科学界。该公司在美国制造商在ISO13485认证,专注于建立长期合作伙伴关系,并利用内部和外部创新的突破性技术商业化。

详细介绍


ProductNamePartNo.UnitSizeConcentrationNotes
NEWPRODUCTS:
E.coliDNALigaseL6090L2,50010,000U/mlEfficientlyligatesDNAatnicksandcohesivetermini.Blunt-endedterminicanbejoinedinthepresenceofPEG
T4RNALigase2L6080L4,50030,000U/mlLigatesnicksondoublestrandedDNAandfromthe3′OHofRNAtothe5′phosphateofDNAindoublestrandedstructures
T4RNALigase2TruncatedL6070L5005,000U/mlRequiresapreadenylated5′phosphatecontainingDNAorRNAtoligateto3′hydroxylofRNA
OmniKlentaq®P7500-HC-L8,40042,000U/mlEngineeredTaqPolymerasemutantwhichenablesPCRdirectfromblood,soil,waterandfoodsamples.Increasedtolerancetofluorescentintercalatingdyes
(HighConcentration)
OmniKlentaq®P7500-LC-L8,4004,200U/mlLowerconcentration–1microliterperreaction
(LowConcentration)
PhoenixHotStartTaqDNAPolymeraseP7590L5005,000U/mlAntibodybasedHotStartTaqDNApolymerasedeliversrobustPCRperformancewithexceptionalpre-PCRcyclingroomtemperaturestability
VeraSeq™2.0High-FidelityDNAPolymeraseP7511L5002,000U/mlIndustry-leadingspeed,fidelityandrobustnessdeliveredviaanengineeredPCRpolymerase
VeraSeq™ULtraDNAPolymeraseP7520L5002,000U/mlHighfidelity,speed,androbustnessinPCRdeliveredbyanengineered,thermostableDNApolymerasethattoleratesuraciltemplates,nucleotidesandprimers
RecAY9260L1.5mg2.0mg/mlPromotesstrandexchangeofsingle-strandedDNAfragmentswithDNAduplexsubstrates
ThermolabilePhosphataseY9210L5,5005,000U/mlRemoves5′and3′-phosphategroupsfromDNA
ThermolabileUDGG5020L5001,000U/mlRemovesuracilfromDNA,leavingAPsite
PolyAPolymeraseP7460L1,0005,000U/mlCatalyzestheadditionofAMPfromATPtothe3′hydroxylofRNAwhichisusefulformakingpolyAtailedRNA
UltraPureLigases:
Bitmap E.coliDNALigaseL6090L2,50010,000U/mlEfficientlyligatesDNAatnicksandcohesivetermini.Blunt-endedterminicanbejoinedinthepresenceofPEG
T3DNALigaseL6010L900,0003,000,000U/mlWorksinhighionicstrength–to1.0MNaCl
T4DNALigaseL6030-LC-L150,000120,000U/mlLowconcentrationovernightligationformat
T4DNALigase(Rapid)L6030-HC-L240,000600,000U/ml“Ultrapureligase”referencedinNatureMethods(Quail,1December2008)
Bitmap T4RNALigase1L6050L10,00020,000U/mlSingle-strandedRNAligase.Alsojoinssingle-strandedDNAmolecules
T4RNALigase2L6080L4,50030,000U/mlLigatesnicksondoublestrandedDNAandfromthe3′OHofRNAtothe5′phosphateofDNAindoublestrandedstructures
Bitmap T4RNALigase2TruncatedL6070L5005,000U/mlRequiresapreadenylated5′phosphatecontainingDNAorRNAtoligateto3′hydroxylofRNA
T7DNALigaseL6020L900,0003,000,000U/ml1000foldhigheractivityonstickyendsthanbluntends
TaqDNALigaseL6060L20,00040,000U/mlThermostableDNAligasewhichefficientlysealsnicksanddiscriminatesagainstmismatchligation
UltraPurePolymerases:
DNAPolymeraseIP7050L5,00010,000U/mlDNApolymerasewith5′→3′synthesis,
5′→3′and3′→5′exonucleaseactivities
Klenow(3′→5′exo-)P7010-HC-L10,00050,000U/mlProvenchoiceforDNAlabeling,A-tailing
Klenow(3′→5′exo-)P7010-LC-L10,0005,000U/mlProvenchoiceforDNAlabeling,A-tailing
KlenowFragmentP7060L2,5005,000U/mlDNApolymeraseusefulforend-fillingpriortoblunt-endligation
MakoDNAPolymeraseP7090L3,00030,000U/mlExonuclease-deficientpolymerase,lacksstranddisplacementactivity
Manta1.0DNAPolymeraseP7140-HC-L100,000400,000U/mlThermostableBacillusDNAPolymerase,strongstranddisplacement
(HighConcentration)
Manta1.0DNAPolymeraseP7140-LC-L100,00040,000U/mlThermostableBacillusDNAPolymerase,strongstranddisplacement
(LowConcentration)
Bitmap MMuLVReverseTranscriptaseP7040L100,000200,000U/mlUsefulpolymeraseforfirst-strandDNAsynthesis
OmniKlentaq®P7500-HC-L8,40042,000U/mlEngineeredTaqPolymerasemutantwhichenablesPCRdirectfromblood,soil,waterandfoodsamples.Increasedtolerancetofluorescentintercalatingdyes
Bitmap (HighConcentration)
OmniKlentaq®P7500-LC-L8,4004,200U/mlLowerconcentration–1microliterperreaction

Enzymatics

Enzymatics专注于于酶产品的研发,为市场带来了特殊的、高品质的产品。是一家试剂、试剂盒和测定的领先生产商,使全球客户变革了诊断、治疗和生命科学的研究。业务包括:试剂、供应链解决方案,应用科学,其测序产品市场占有率达80%。酶科技公司产品包括八类:超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(ArcherProducts)、连接蛋白、DNA修饰酶、NGS文库制备(NGSLibraryConstruction)、核酸和RNA酶。2015年1月,QIAGEN收购Enzymatics。 收起
试剂

货号:EnzymaticsY9040L

品名:T4PolynucleotideKinase(T4多核苷酸激酶)

产品描述:

T4多核苷酸激酶(PNK)催化ATP的末端γ位磷酸酯转移和交换到双链和单链DNA,RNA和核苷3'-单磷酸酯分子的5'-羟基末端(1)。T4PNK还表现出3'-磷酸酶和2',3'环磷酸二酯酶活性(2,3,4,5,6)。

 

规格:

10,000U

浓度

10,000U/ml

保存

-25°C至-15°C

纯度(SDS-PAGE)

>99%

活性:

133,333U/mg

SS核酸外切酶

2000U<5.0%released

DS核酸外切酶

2000U<1.0%released

DS内切核酸酶

2000U=Noconversion

大肠杆菌 DNA污染

2000U<10copies

 

蛋白质来源

从表达重组T4多核苷酸激酶基因的大肠杆菌中纯化而得。

 

补充:

10mMTris-HCl

50mMKCl

0.1碌MATP

1.0mMDTT

0.1mMEDTA

50%glycerol

pH7.4@25掳C

 

随附:

B9040(10XT4PolynucleotideKinaseBuffer):

 

10X多核苷酸激酶缓冲液(B9040)700mM

Tris-HCl 

100mMMgCl 2

50mMDTT 

pH7.6在25℃

 

单位定义
一个单位定义为在1XT4多核苷酸激酶反应缓冲液中,37℃下30分钟内催化掺入1nmol[ 32 P](ATP供体)的酶量。

 

质量控制分析

 

单位表征测定
单位活性使用2倍系列稀释法测量。在1X反应缓冲液中制备酶的稀释液,并将其加入到50μL含有10μMOligodT单链DNA,1XPNK反应缓冲液和66μMATP的反应中,并将[γ- 32 P]ATP反应在37°孵育30分钟将其置于冰上,并使用Sambrook和Russell的方法(MolecularCloning,v3,2001,pp.A8.25-A8.26)进行分析。

 

蛋白质浓度(OD 280)测量使用使用2.0mg/mlBSA样品(PierceCat#23209)标准化的NanodropND-1000分光光度计
在OD 280处分析2.0μL酶样品并用产品储存溶液冲切。使用消光系数63,440和分子量34,620道尔顿将观察到的3个重复样品的平均测量结果转换为mg/mL。

 

SDS-Page(物理纯度评估)
将2.0μL酶溶液加载到变性的4-20%Tris-甘氨酸SDS-PAGE凝胶上,其侧翼为宽范围MW标记和2.0μL1:100稀释的样品。电泳后,将凝胶染色并比较样品以确定物理纯度。该测试的验收标准要求浓缩样品中污染带的总量不超过稀释样品中目的蛋白质条带的质量,确认浓缩样品的纯度大于99%。

 

 

部分产品信息:

货号

品名

规格

浓度

品牌

N2070-10-L

ATPSolution 

50µmol

10mM

Enzymatics

N2050-10-L

10mMdNTPSolutionMix

100µmol

10mM

Enzymatics

N2010L

100mM75µmoldNTPSolutionSet 

75µmol

100mM

Enzymatics

N2010-A-L

100mMdATPSolution 

75µmol

100mM

Enzymatics

N2050L

100µmoldNTPSolutionMix 

100µmol

25mM

Enzymatics

Y9180L

10xUracilCleavageSystem 

750Reactions

N/A

Enzymatics

P7670

2xHiFiPCRMasterMix 

1rxn

2x

Enzymatics

Y9420L

5xER/A-TailingEnzymeMix 

24reactions

N/A

Enzymatics

Y9380L

E.coli Pyrophosphatase 

50U

100U/mL

Enzymatics

Y9030L

E.coliSingle-StrandedDNABindingProtein 

1.5mg

2.75mg/ml

Enzymatics

Y9140-HC-L

End-RepairMix(HighConcentration) 

75Reactions

1Rxn/µL

Enzymatics

Y9140-LC-L

End-RepairMix(LowConcentration) 

200Reactions

1Rxn/µL

Enzymatics

Y9080L

EndonucleaseVIII 

10,000U

10,000U/ml

Enzymatics

P7600L

EnzScript™(MMLVReverseTranscriptaseRNaseH-) 

10,000U

200,000U/mL

Enzymatics

X8010L

ExonucleaseI 

30,000U

20,000U/ml

Enzymatics

X8020L

ExonucleaseIII 

50,000U

100,000U/ml

Enzymatics

P7010-HC-L

Klenow(3′→5′exo-)(HighConcentration) 

10,000U

50,000U/ml

Enzymatics

P7010-LC-L

Klenow(3′→5′exo-)(LowConcentration) 

10,000U

5,000U/ml

Enzymatics

P7060L

KlenowFragment 

2,500U

5,000U/ml

Enzymatics

X8030L

LambdaExonuclease 

10,000U

5,000U/ml

Enzymatics

P7040L

M-MuLVReverseTranscriptase 

100,000U

200,000U/ml

Enzymatics

P7090L

MakoDNAPolymerase(3′→5′exo-) 

3,000U

30,000U/ml

Enzymatics

P7140-HC-L

Manta1.0DNAPolymerase(HighConcentration) 

100,000U

400,000U/ml

Enzymatics

P7140-LC-L

Manta1.0DNAPolymerase(LowConcentration) 

100,000U

40,000U/ml

Enzymatics

P7610L

NGSLibraryPrep:2XVeraSeq™PCRMix–250Reactions 

250Reactions

2X

Enzymatics

P7590L

Phoenix™HotStartTaqDNAPolymerase 

500U

5,000U/ml

Enzymatics

P7460L

Poly(A)Polymerase 

1,000U

5,000U/mL

Enzymatics

Y9260L

RecA 

1.5mg

2.0mg/mL

Enzymatics

Y9220L

RNAseH 

5,000U

5,000U/ml

Enzymatics

Y9240L

RNAseInhibitor 

20,000U

40,000U/ml

Enzymatics

L6010L

T3DNALigase 

900,000U

3,000,000U/ml

Enzymatics

L6030-LC-L

T4DNALigase 

150,000U

120,000U/ml

Enzymatics

L6030-HC-L

T4DNALigase(Rapid) 

240,000U

600,000U/ml

Enzymatics

P7080L

T4DNAPolymerase 

2,000U

3,000U/ml

Enzymatics

Y9130L

T4Gene32Protein 

1.0mg

10mg/mL

Enzymatics

Y9040L

T4PolynucleotideKinase 

10,000U

10,000U/ml

Enzymatics

L6050L

T4RNALigase1 

10,000U

20,000U/ml

Enzymatics

L6080L

T4RNALigase2 

4,500U

30,000U/ml

Enzymatics

L6070L

T4RNALigase2Truncated 

500U

5,000U/ml

Enzymatics

L6020L

T7DNALigase 

900,000U

3,000,000U/ml

Enzymatics

P7260L

T7DNAPolymerase 

3,500U

10,000U/ml

Enzymatics

P7180L

T7RNAPolymerase 

50,000U

50,000U/ml

Enzymatics

L6060L

TaqDNALigase 

20,000U

40,000U/ml

Enzymatics

P7110L

TaqDSC2.0DNAPolymerase 

1,250U

5,000U/ml

Enzymatics

P7250L

Taq-BDNAPolymerase 

10,000U

5,000U/ml

Enzymatics

P7620L

TaqIT 

5,000U

50,000U/mL

Enzymatics

P7070L

TerminaldeoxynucleotidylTransferase(TdT) 

6,000U

20,000U/ml

Enzymatics

G5020L

ThermolabileUDG 

500U

1,000U/ml

Enzymatics

Y9370L

ThermostablePyrophosphatase 

1,250U

2,000U/ml

Enzymatics

G5010L

UracilDNAGlycosylase 

10,000U

2,000U/ml

Enzymatics

P7511L

VeraSeq™2.0High-FidelityDNAPolymerase 

500U

2,000U/ml

Enzymatics

P7520L

VeraSeq™ULtraDNAPolymerase 

500U

2,000U/ml

Enzymatics

L6030-W-L

WGSLigase 

24Reactions

N/A

Enzymatics

P7640L

ZipScript™One-StepRT-qPCRKit 

1000Reactions

N/A

Enzymatics

P7020-HC-L

φ29DNAPolymerase(HighConcentration) 

2,000U

100,000U/ml

Enzymatics

P7020-LC-L

φ29DNAPolymerase(LowConcentration) 

2,000U

10,000U/ml

Enzymatics

 


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